Blood coagulant is a suspended liquid mainly composed of thrombin, alcohol, silica powder, emulsifier, surfactant, etc. Vacuum and injection type blood collection tube can be made and applied in clinical medical laboratory work. Laboratory personnel can complete operations such as taking blood samples, quickly separating serum, and transmitting samples in the same tube. 

1. Blood coagulation is related to factors such as environmental temperature, degree of reverse mixing, patient blood characteristics, coagulant performance, and blood collection volume, with the greatest correlation being with temperature. When the temperature of the blood collection environment is below 20 ℃, it is necessary to increase the dosage of coagulant appropriately, increase the frequency of reverse mixing, extend the coagulation time, or use it in conjunction with a 36 ℃ water bath. 

2. Insufficient coagulation can cause blood cells and fibrin filaments to hang on the wall, as well as mild hemolysis. Some patients may experience the interruption of coagulation process, collagen precipitation, or no serum separation after centrifugation when their blood is not completely rounded at one time. 

3. The coagulation time is 5 to 15 minutes before centrifugation, which may cause secondary precipitation of fibrin filaments, resulting in fibrin filaments floating in the serum and easily causing probe blockage. 

4. When coagulants are used together with separation gel, poor coagulation effect can affect serum quality and separation gel isolation effect. 

5. Suggestion for irradiation sterilization after adding to blood collection tubes: use γ Radiation irradiation, with a dose of 8-250KGy. The irradiation dose is controlled based on the initial colony count. 

6. It is recommended not to directly touch the coagulant with your hands. When it sticks to the skin, it can be rinsed with water.